Journal:

Article Title: Target Cell Range of Haemophilus ducreyi Hemolysin and Its Involvement in Invasion of Human Epithelial Cells

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: The bacterial strains and plasmids used in this study are described in Table and Fig. . Human cell lines were obtained from the American Type Culture Collection (Manassas, Va.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain or plasmid Relevant characteristics Reference or source Strains E. coli HB101 Noninvasive strain, negative control 18 S. typhimurium SL1344 Highly invasive strain, positive control 16 H. influenzae Rd Nonhemolytic, noncytotoxic, negative control, ATCC 33391 M. Roberts, University of Washington H. ducreyi 35000 Wild type, hemolytic 41 35000ΔAPC 35000 hhdA :: cat , nonhemolytic, Cm r This study Plasmids pPT376 pUC19 with hhdA , Ap r 41 pBluescript KS(−) Cloning vector, Ap r Stratagene pBluescript SK(−) Cloning vector, Ap r Stratagene pPT376KS hhdA on pBluescript, Ap r This study pPT376ΔPst pPT376KS with internal hhdA Pst I fragment replaced with Bam HI linkers, Ap r This study pUC-ΔE cat Source of cat cassette, Cm r B.

Techniques: Plasmid Preparation, Negative Control, Positive Control, Cloning, TA Cloning